Differential transcriptomic responses of Biomphalaria glabrata (Gastropoda, Mollusca) to bacteria and metazoan parasites, Schistosoma mansoni and Echinostoma paraensei (Digenea, Platyhelminthes).
Mol Immunol, 2010/1;47(4):849-60.
Adema CM[1], Hanington PC, Lun CM, Rosenberg GH, Aragon AD, Stout BA, Lennard Richard ML, Gross PS, Loker ES
Affiliations
PMID: 19962194DOI: 10.1016/j.molimm.2009.10.019
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Abstract
A 70-mer-oligonucleotide-based microarray (1152 features) that emphasizes stress and immune responses factors was constructed to study transcriptomic responses of the snail Biomphalaria glabrata to different immune challenges. In addition to sequences with relevant putative ID and Gene Ontology (GO) annotation, the array features non-immune factors and unknown B. glabrata ESTs for functional gene discovery. The transcription profiles of B. glabrata (3 biological replicates, each a pool of 5 snails) were recorded at 12h post-wounding, exposure to Gram negative or Gram positive bacteria (Escherichia coli and Micrococcus luteus, respectively), or infection with compatible trematode parasites (Schistosoma mansoni or Echinostoma paraensei, 20 miracidia/snail), relative to controls, using universal reference RNA. The data were subjected to Significance Analysis for Microarrays (SAM), with a false positive rate (FPR)
MeSH terms
Animals; Bacteria; Biomphalaria; Echinostoma; Gene Expression Profiling; Gene Expression Regulation; Oligonucleotide Array Sequence Analysis; Parasites; RNA, Messenger; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Schistosoma mansoni; Stress, Physiological; Time Factors; Transcription, Genetic
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