Home
/
Data resources
/
Literature
/
19955278
Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies.

J Clin Microbiol, 2010/2;48(2):545-53.

Kim HS[1], Lee DS, Chang YH, Kim MJ, Koh S, Kim J, Seong JH, Song SK, Shin HS, Son JB, Jung MY, Park SN, Yoo SY, Cho KW, Kim DK, Moon S, Kim D, Choi Y, Kim BO, Jang HS, Kim CS, Kim C, Choe SJ, Kook JK

Affiliations

PMID: 19955278DOI: 10.1128/JCM.01631-09

Impact factor: 11.677

Abstract
Fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. fusiforme, F. nucleatum subsp. vincentii, and F. nucleatum subsp. animalis) based on several phenotypic characteristics and DNA-DNA hybridization patterns. However, the methods for detecting or discriminating the clinical isolates of F. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. Therefore, in this study, the nucleotide sequences of the RNA polymerase beta-subunit gene (rpoB) and zinc protease gene were analyzed to discriminate the subspecies of F. nucleatum. The partial sequences of rpoB (approximately 2,419 bp), the zinc protease gene (878 bp), and 16S rRNA genes (approximately 1,500 bp) of the type strains of five subspecies, 28 clinical isolates of F. nucleatum, and 10 strains of F. periodonticum (as a control group) were determined and analyzed. The phylogenetic data showed that the rpoB and zinc protease gene sequences clearly delineated the subspecies of F. nucleatum and provided higher resolution than the 16S rRNA gene sequences in this respect. According to the phylogenetic analysis of rpoB and the zinc protease gene, F. nucleatum subsp. vincentii and F. nucleatum subsp. fusiforme might be classified into a single subspecies. Five clinical isolates could be delineated as a new subspecies of F. nucleatum. The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.
MeSH terms
Bacterial Proteins; Bacterial Typing Techniques; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; DNA-Directed RNA Polymerases; Fusobacterium Infections; Fusobacterium nucleatum; Genotype; Humans; Metalloendopeptidases; Molecular Sequence Data; Phylogeny; Polymorphism, Genetic; RNA, Ribosomal, 16S; Sequence Analysis, DNA
More resources
EndNote: Download