Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
Nat Methods, 2010/1;7(1):47-9.
Goren A[1], Ozsolak F, Shoresh N, Ku M, Adli M, Hart C, Gymrek M, Zuk O, Regev A, Milos PM, Bernstein BE
Affiliations
PMID: 19946276DOI: 10.1038/nmeth.1404
Impact factor: 47.99
Abstract
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.
MeSH terms
Animals; Bias; Chromatin; Chromatin Immunoprecipitation; Gene Expression Profiling; Genome; Humans; Mice; Sequence Analysis, DNA; Transcription Factors
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