Detection of novel swine origin influenza A virus (H1N1) by real-time nucleic acid sequence-based amplification.
J Virol Methods, 2010/2;163(2):495-7.
Ge Y[1], Cui L, Qi X, Shan J, Shan Y, Qi Y, Wu B, Wang H, Shi Z
Affiliations
PMID: 19883690DOI: 10.1016/j.jviromet.2009.10.025
Impact factor: 2.623
Abstract
Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assay was evaluated and validated by comparing it with existing detection methods for S-OIV (H1N1). Results obtained in a 10-fold dilution series assay demonstrated the analytic sensitivity of the present assay was comparable to that of a commercial S-OIV (H1N1) real-time RT-PCR kit and higher than that of the Centers for Disease Control and Prevention (CDC) TaqMan assay. The actual detection limit of the real-time NASBA assay was approximately 50 copies per reaction. Compared with reference methods (viral culture, conventional RT-PCR, and real-time RT-PCR), the sensitivity, specificity, positive predictive value, and negative predictive value of the present assay were all 100%. Overall, the results showed that the real-time NASBA assay could be used for sensitive and specific detection of S-OIV (H1N1).
MeSH terms
Hemagglutinins, Viral; Humans; Influenza A Virus, H1N1 Subtype; Influenza, Human; Predictive Value of Tests; Self-Sustained Sequence Replication; Sensitivity and Specificity
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