Generation of functional ventricular heart muscle from mouse ventricular progenitor cells.
Science, 2009/10/16;326(5951):426-9.
Domian IJ[1], Chiravuri M, van der Meer P, Feinberg AW, Shi X, Shao Y, Wu SM, Parker KK, Chien KR
Affiliations
PMID: 19833966DOI: 10.1126/science.1177350
Impact factor: 63.714
Abstract
The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.
MeSH terms
Action Potentials; Animals; Cell Cycle; Cell Differentiation; Cell Line; Cell Lineage; Cells, Cultured; Embryonic Stem Cells; Gene Expression; Heart; Heart Ventricles; Mice; Mice, Transgenic; Muscle Development; Myocardial Contraction; Myocytes, Cardiac; Oligonucleotide Array Sequence Analysis; Tissue Engineering; Ventricular Function
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