Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription.
Proc Natl Acad Sci U S A, 2009/8/25;106(34):14265-70.
Tavenet A[1], Suleau A, Dubreuil G, Ferrari R, Ducrot C, Michaut M, Aude JC, Dieci G, Lefebvre O, Conesa C, Acker J
Affiliations
PMID: 19706510DOI: 10.1073/pnas.0900162106
Impact factor: 12.779
Abstract
Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a system reconstituted with all recombinant factors. Sub1 was required for optimal Pol III gene transcription in exponentially growing cells.
MeSH terms
Blotting, Far-Western; Chromatin Immunoprecipitation; Chromosome Mapping; Chromosomes, Fungal; DNA, Fungal; DNA-Binding Proteins; Gene Expression Regulation, Developmental; Gene Expression Regulation, Fungal; Genome, Fungal; Mutation; Oligonucleotide Array Sequence Analysis; Protein Binding; RNA Polymerase III; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription Factors, TFIII; Transcription, Genetic
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download