Carotenoid production in Bacillus subtilis achieved by metabolic engineering.
Biotechnol Lett, 2009/11;31(11):1789-93.
Affiliations
PMID: 19618272DOI: 10.1007/s10529-009-0082-6
Impact factor: 2.716
Abstract
The carotenoid synthetic genes, crtM and crtN, derived from Staphylococcus aureus, were introduced into B. subtilis, resulting in yellow pigmentation. Absorption maxima of pigments and MALDI-TOF mass spectrometry demonstrated that the pigmented strain accumulated two C(30) carotenoids, 4,4'-diapolycopene and 4,4'-diaponeurosporene. A survival test using H(2)O(2) revealed that the pigmented strain was more resistant to oxidative stress than the strain harboring an empty-vector. These findings indicate that B. subtilis can produce carotenoids, and the strain accumulating the carotenoids, CarotenoBacillus, will become a basal host for production of C(30) carotenoids and evaluation of their antioxidative effects.
MeSH terms
Bacillus subtilis; Carotenoids; Chromatography, High Pressure Liquid; Genetic Engineering; Hydrogen Peroxide; Mass Spectrometry; Microbial Viability; Staphylococcus aureus
More resources
EndNote: Download