Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes.
FEBS Lett, 2009/7/21;583(14):2377-84.
Affiliations
PMID: 19555691DOI: 10.1016/j.febslet.2009.06.035
Impact factor: 3.864
Abstract
To compare gene expression profiles in response to estrogen or 17beta-estradiol (E(2)) and a mycotoxin, zearalenone (ZEA), and its analogues (collectively termed ZEA compounds), breast cancer MCF-7 cells were treated with 10 nM of E(2) or ZEA compounds including ZEA, alpha-zearalenol, beta-zearalenol, zearalanone, alpha-zearalanol and beta-zearalanol. Expression profiles for 120 estrogen-responsive genes were subjected to cluster and statistical analyses using correlation coefficients or R-values. We found that all of the ZEA compounds stimulated the growth of MCF-7 cells, as much as E(2), and showed similar expression profiles to that of E(2) (R-values ranged from 0.82 to 0.96). The effect of ZEA compounds was likely mediated by estrogen-receptor-dependent Erk1/2-signaling. These results provide clues to understand the mechanism of their estrogen-like action.
MeSH terms
Breast Neoplasms; Cell Line, Tumor; Dose-Response Relationship, Drug; Enzyme Activation; Estradiol; Estrogens; Estrogens, Non-Steroidal; Extracellular Signal-Regulated MAP Kinases; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Molecular Structure; Oligonucleotide Array Sequence Analysis; Receptors, Estrogen; Signal Transduction; Zearalenone
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