Limited transcriptional response of ovine microglia to prion accumulation.
Biochem Biophys Res Commun, 2009/8/21;386(2):345-50.
Stanton JB[1], Knowles DP, Call DR, Mathison BA, Baszler TV
Affiliations
PMID: 19523453DOI: 10.1016/j.bbrc.2009.06.030
Impact factor: 3.322
Abstract
The conversion of normal cellular prion protein to disease-associated prion protein (PrP(Sc)) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP(Sc) accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP(Sc) accumulation in cultured cells, the transcriptional profile of PrP(Sc)-accumulating primary ovine microglia was compared to the profile of PrP(Sc)-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP(Sc) accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP(Sc)-accumulating microglia. The results support the hypothesis that chronic PrP(Sc) accumulation in cultured microglia results in a limited transcriptional response.
MeSH terms
Animals; Cattle; Gene Expression Profiling; Microglia; Oligonucleotide Array Sequence Analysis; PrPSc Proteins; Sheep; Transcription, Genetic
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download