Cryptococcus bromeliarum sp. nov., an orange-coloured basidiomycetous yeast isolated from bromeliads in Brazil.
Int J Syst Evol Microbiol, 2009/4;59(Pt 4):910-3.
Landell MF[1], Inácio J, Fonseca A, Vainstein MH, Valente P
Affiliations
PMID: 19329627DOI: 10.1099/ijs.0.005652-0
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Abstract
During a survey of yeasts associated with the phylloplane of several bromeliad species in Itapuã Park in southern Brazil, we isolated four orange-coloured strains which were found to represent a novel anamorphic tremellaceous (Tremellales, Agaricomycotina, Basidiomycota) yeast species, Cryptococcus bromeliarum sp. nov. (type strain BI20(T) =CBS 10424(T) =NRRL Y-48112(T)). PCR-fingerprinting profiles of the four strains with primers M13 and (GTG)(5) were almost identical, which suggested conspecificity among the isolates. On the basis of D1/D2 26S rDNA sequence analysis, C. bromeliarum is phylogenetically closely related to other orange-coloured Cryptococcus species, namely Cryptococcus armeniacus, C. amylolyticus, C. tibetensis and C. cistialbidi, but differed from these species by at least six nucleotide substitutions and was thus considered a separate species. Physiological differences from C. armeniacus, C. amylolyticus and C. cistialbidi included the inability of C. bromeliarum to assimilate citrate and to form starch-like compounds. Differentiation from C. tibetensis can be achieved by the ability of the latter to assimilate ethylamine.
MeSH terms
Brazil; Bromelia; Cryptococcus; DNA Fingerprinting; DNA, Fungal; DNA, Ribosomal; Genes, rRNA; Molecular Sequence Data; Mycological Typing Techniques; Phylogeny; Pigments, Biological; Plant Leaves; RNA, Fungal; RNA, Ribosomal; Sequence Analysis, DNA
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