Variation in the replication loci of Streptomyces linear plasmids.
FEMS Microbiol Lett, 2009/1;290(2):209-16.
Zhang R[1], Xia H, Guo P, Qin Z
Affiliations
PMID: 19054078DOI: 10.1111/j.1574-6968.2008.01432.x
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Abstract
Streptomyces linear plasmids start replication at centrally located loci, usually consisting of iterons and adjacent rep genes. Here, we identified four new replication loci from Streptomyces linear plasmids. A discontinuous locus, consisting of two genes and iterons separated by two nonessential genes, was required for replication of pRL2 in both linear and circular modes. A temperature-sensitive plasmid, pRL4, contained a replication locus, a noncoding sequence and a SAP1.35-like gene. A telomere-adjacent locus, another noncoding sequence and SAP1.1-like gene, was identified for replication of the large plasmid pFRL2. The replication locus of pSHK1 consisted of SCP1-rep-like genes and iterons. These results indicate an unexpected variety of components, positions and combinations of replication loci among Streptomyces linear plasmids.
MeSH terms
DNA Replication; Genetic Variation; Molecular Sequence Data; Plasmids; Replication Origin; Streptomyces
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