The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome.
Proc Natl Acad Sci U S A, 2008/12/02;105(48):18964-9.
Sela DA[1], Chapman J, Adeuya A, Kim JH, Chen F, Whitehead TR, Lapidus A, Rokhsar DS, Lebrilla CB, German JB, Price NP, Richardson PM, Mills DA
Affiliations
PMID: 19033196DOI: 10.1073/pnas.0809584105
Impact factor: 12.779
Abstract
Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated by bifidobacteria. Accordingly, the complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects a competitive nutrient-utilization strategy targeting milk-borne molecules which lack a nutritive value to the neonate. Several chromosomal loci reflect potential adaptation to the infant host including a 43 kbp cluster encoding catabolic genes, extracellular solute binding proteins and permeases predicted to be active on milk oligosaccharides. An examination of in vivo metabolism has detected the hallmarks of milk oligosaccharide utilization via the central fermentative pathway using metabolomic and proteomic approaches. Finally, conservation of gene clusters in multiple isolates corroborates the genomic mechanism underlying milk utilization for this infant-associated phylotype.
MeSH terms
Bacterial Proteins; Bifidobacterium; Breast Feeding; Female; Gastrointestinal Tract; Genome, Bacterial; Humans; Infant, Newborn; Milk, Human; Molecular Sequence Data; Multigene Family; Oligosaccharides; Phylogeny; Pregnancy
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