The complete nucleotide sequences of the 5 genetically distinct plastid genomes of Oenothera, subsection Oenothera: II. A microevolutionary view using bioinformatics and formal genetic data.
Mol Biol Evol, 2008/9;25(9):2019-30.
Greiner S[1], Wang X, Herrmann RG, Rauwolf U, Mayer K, Haberer G, Meurer J
Affiliations
PMID: 18614526DOI: 10.1093/molbev/msn149
Impact factor: 8.8
Abstract
A unique combination of genetic features and a rich stock of information make the flowering plant genus Oenothera an appealing model to explore the molecular basis of speciation processes including nucleus-organelle coevolution. From representative species, we have recently reported complete nucleotide sequences of the 5 basic and genetically distinguishable plastid chromosomes of subsection Oenothera (I-V). In nature, Oenothera plastid genomes are associated with 6 distinct, either homozygous or heterozygous, diploid nuclear genotypes of the 3 basic genomes A, B, or C. Artificially produced plastome-genome combinations that do not occur naturally often display interspecific plastome-genome incompatibility (PGI). In this study, we compare formal genetic data available from all 30 plastome-genome combinations with sequence differences between the plastomes to uncover potential determinants for interspecific PGI. Consistent with an active role in speciation, a remarkable number of genes have high Ka/Ks ratios. Different from the Solanacean cybrid model Atropa/tobacco, RNA editing seems not to be relevant for PGIs in Oenothera. However, predominantly sequence polymorphisms in intergenic segments are proposed as possible sources for PGI. A single locus, the bidirectional promoter region between psbB and clpP, is suggested to contribute to compartmental PGI in the interspecific AB hybrid containing plastome I (AB-I), consistent with its perturbed photosystem II activity.
MeSH terms
Base Sequence; Chloroplasts; Chromosome Mapping; DNA, Intergenic; DNA, Plant; Evolution, Molecular; Expressed Sequence Tags; Genetic Speciation; Genome, Plant; Genotype; Oenothera; RNA Editing; Selection, Genetic
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download