Gene expression profiling of human mesenchymal stem cells for identification of novel markers in early- and late-stage cell culture.
J Biochem, 2008/9;144(3):399-408.
Tanabe S[1], Sato Y, Suzuki T, Suzuki K, Nagao T, Yamaguchi T
Affiliations
PMID: 18550633DOI: 10.1093/jb/mvn082
Impact factor: 3.241
Abstract
Human mesenchymal stem cells (hMSCs) are multipotent cells that differentiate into several cell types, and are expected to be a useful tool for cellular therapy. Although the hMSCs differentiate into osteogenic cells during early to middle stages, this differentiation capacity decreases during the late stages of cell culture. To test a hypothesis that there are biomarkers indicating the differentiation potential of hMSCs, we performed microarray analyses and profiled the gene expression in six batches of hMSCs (passages 4-28). At least four genes [necdin homolog (mouse) (NDN), EPH receptor A5 (EPHA5), nephroblastoma overexpressed gene (NOV) and runt-related transcription factor 2 (RUNX2)] were identified correlating with the passage numbers in all six batches. The results showed that the osteogenic differentiation capacity of hMSCs is down-regulated in the late stages of cell culture. It seemed that adipogenic differentiation capacity was also down-regulated in late stage of the culture. The cells in late stage are oligopotent and the genes identified in this study have the potential to act as quality-control markers of the osteogenic differentiation capacity of hMSCs.
MeSH terms
Adipocytes; Bone Marrow Cells; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Connective Tissue Growth Factor; Core Binding Factor Alpha 1 Subunit; Gene Expression Profiling; Gene Expression Regulation; Genetic Markers; Humans; Immediate-Early Proteins; Intercellular Signaling Peptides and Proteins; Mesenchymal Stem Cells; Nephroblastoma Overexpressed Protein; Nerve Tissue Proteins; Nuclear Proteins; Oligonucleotide Array Sequence Analysis; Osteogenesis
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