Detection and molecular characterisation of plasmidic AmpC beta-lactamases in Klebsiella pneumoniae isolates from a tertiary-care hospital in Dublin, Ireland.
Clin Microbiol Infect, 2008/6;14(6):616-8.
Roche C[1], Boo TW, Walsh F, Crowley B
Affiliations
PMID: 18397332DOI: 10.1111/j.1469-0691.2008.01998.x
Impact factor: 13.31
Abstract
This study determined the types of AmpC enzymes produced by Klebsiella pneumoniae isolates resistant to third-generation cephalosporins and the clonality of these isolates. The presence of AmpC enzymes was identified by cephalosporin-cloxacillin synergy tests. Genes encoding AmpC enzymes were characterised by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) was used to type the isolates. Fifteen K. pneumoniae isolates were positive for bla(AmpC), 13 were positive for bla(ACC-1) and two were positive for bla(DHA-1). Production of the DHA-1 enzyme was inducible. The ampR gene was identified upstream of the bla(DHA-1) gene. PFGE demonstrated the polyclonal origin of the isolates carrying bla(ACC-1).
MeSH terms
Anti-Bacterial Agents; Bacterial Proteins; Cephalosporin Resistance; Cephalosporins; Cluster Analysis; Cross Infection; Electrophoresis, Gel, Pulsed-Field; Genes, Bacterial; Genotype; Ireland; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Plasmids; Polymerase Chain Reaction; Prevalence; Sequence Analysis, DNA; beta-Lactamases
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