Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos.
EMBO Rep, 2008/5;9(5):465-71.
Stramer B[1], Winfield M, Shaw T, Millard TH, Woolner S, Martin P
Affiliations
PMID: 18344972DOI: 10.1038/embor.2008.34
Impact factor: 9.071
Abstract
By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
MeSH terms
Animals; Drosophila; Drosophila Proteins; Embryo, Nonmammalian; GATA Transcription Factors; Gene Expression; Green Fluorescent Proteins; Hemocytes; Homozygote; Intracellular Signaling Peptides and Proteins; Macrophages; Mutation; Oligonucleotide Array Sequence Analysis; Up-Regulation; Wounds and Injuries; GADD45 Proteins
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