Characterization of the IncA/C plasmid pCC416 encoding VIM-4 and CMY-4 beta-lactamases.
J Antimicrob Chemother, 2007/8;60(2):258-62.
Colinon C[1], Miriagou V, Carattoli A, Luzzaro F, Rossolini GM
Affiliations
PMID: 17540674
Impact factor: 5.758
Abstract
objectives: To characterize the antibiotic resistance regions of pCC416, a VIM-4- and CMY-4-encoding plasmid from clinical enterobacteria, and to elucidate its relation with the CMY-encoding plasmids widely diffused in Salmonella.
methods: The enterobacterial multiresistant plasmid pCC416 was derived from an Escherichia coli transconjugant and characterized. Conventional and long-range PCR assays were performed using primers specific for VIM-4- and CMY-4-encoding segments of pCC416. Amplicons were characterized by sequencing. blaVIM-4, blaCMY-4 and IntI1-specific probes were prepared from PCR products and used for the identification of various pCC416 clones. VIM- and CMY-positive BamHI and Sau3AI fragments of pCC416 were cloned into pACYC184 and their sequences were determined by gene walking.
results: The pCC416 plasmid contained two distinct resistant loci carrying beta-lactamase genes. The blaVIM-4 gene was part of an integron located in a complex, multidrug-resistant region of novel structure, interspersed with mobile elements or remnants thereof and being similar to various regions of other resistance plasmids. Nevertheless, a region in the 3' end of this structure resembled the respective region found in a CMY-2-encoding plasmid from Salmonella. The blaCMY-4 gene was identified within an 11.3 kb region also related to the CMY-2-encoding plasmids.
conclusions: pCC416 probably evolved from an IncA/C2, CMY-encoding plasmid through acquisition of a VIM-encoding In4-type integron providing an example of accretion of resistance determinants in a single replicon.
MeSH terms
Cloning, Molecular; DNA Transposable Elements; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae; Escherichia coli; Microbial Sensitivity Tests; Molecular Sequence Data; Plasmids; Polymorphism, Restriction Fragment Length; Reverse Transcriptase Polymerase Chain Reaction; beta-Lactamases
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