Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera).
Genome Biol, 2006;7(3):R21.
Sabater-Muñoz B[1], Legeai F, Rispe C, Bonhomme J, Dearden P, Dossat C, Duclert A, Gauthier JP, Ducray DG, Hunter W, Dang P, Kambhampati S, Martinez-Torres D, Cortes T, Moya A, Nakabachi A, Philippe C, Prunier-Leterme N, Rahbé Y, Simon JC, Stern DL, Wincker P, Tagu D
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PMID: 16542494
Impact factor: 17.906
Abstract
Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from the pea aphid Acyrthosiphon pisum was carried out to define a catalog of 12,082 unique transcripts. A strong AT bias was found, indicating a compositional shift between Drosophila melanogaster and A. pisum. An in silico profiling analysis characterized 135 transcripts specific to pea-aphid tissues (relating to bacteriocytes and parthenogenetic embryos). This project is the first to address the genetics of the Hemiptera and of a hemimetabolous insect.
MeSH terms
Animals; Aphids; Base Composition; Base Sequence; DNA; DNA, Complementary; Expressed Sequence Tags; Gene Library; Microsatellite Repeats; Pisum sativum; Phylogeny; Plant Diseases; Population Density; Transcription, Genetic
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