Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan.
Clin Microbiol Infect, 2005/11;11(11):893-7.
Wu LT[1], Hung SW, Chuang YC, Chen HE, Jones RN, Yu WL
Affiliations
PMID: 16216104
Impact factor: 13.31
Abstract
A strain of Klebsiella pneumoniae resistant to cefoxitin and oxyimino-cephalosporins, but susceptible to cefepime, was isolated from an adult patient hospitalised in Taichung, Taiwan. Isoelectric focusing revealed three beta-lactamases with isoelectric points of 5.4, 8.2 and 7.9, respectively. Following PCR with plasmid DNA templates and gene sequencing, these enzymes were shown to correspond to TEM-1, SHV-5 and a novel DHA-1-like enzyme (designated DHA-3). The bla genes for TEM-1 and SHV-5 were transferable, but the bla(DHA-3) gene was non-self-transferable in conjugation experiments. All three bla genes were successfully introduced by electrotransformation into an Escherichia coli recipient (DH5alpha), resulting in a similar resistance profile to that observed in the original donor strain. Other K. pneumoniae strains producing DHA-1-like enzymes have been identified previously in Taiwan, and this report suggests that DHA-type beta-lactamases are continuing to emerge in this country.
MeSH terms
Adult; Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Cefoxitin; Cephalosporinase; Conjugation, Genetic; Drug Resistance, Bacterial; Escherichia coli; Gene Transfer, Horizontal; Genes, Bacterial; Humans; Isoelectric Point; Klebsiella Infections; Klebsiella pneumoniae; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Sequence Analysis, DNA; Taiwan
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