Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.
Genome Biol, 2005;6(7):R61.
Carter MG[1], Sharov AA, VanBuren V, Dudekula DB, Carmack CE, Nelson C, Ko MS
Affiliations
PMID: 15998450
Impact factor: 17.906
Abstract
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.
MeSH terms
Animals; Base Sequence; Expressed Sequence Tags; Gene Expression Profiling; Genome; Introns; Mice; Models, Genetic; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Open Reading Frames; RNA, Messenger; Restriction Mapping; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae; Transcription, Genetic
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