Mutagenesis analysis of a conserved region involved in acetyl coenzyme A binding in the aminoglycoside 6'-N-acetyltransferase type Ib encoded by plasmid pJHCMW1.
Antimicrob Agents Chemother, 2005/7;49(7):2979-82.
Pourreza A[1], Witherspoon M, Fox J, Newmark J, Bui D, Tolmasky ME
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PMID: 15980378
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Abstract
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.
MeSH terms
Acetyl Coenzyme A; Acetyltransferases; Alanine; Amikacin; Amino Acid Sequence; Anti-Bacterial Agents; Conserved Sequence; DNA, Bacterial; Escherichia coli; Kanamycin; Microbial Sensitivity Tests; Models, Molecular; Molecular Sequence Data; Mutagenesis; Plasmids
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