Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida.
Lett Appl Microbiol, 2005;40(6):453-9.
Margesin R[1], Fauster V, Fonteyne PA
Affiliations
PMID: 15892742
Impact factor: 2.813
Abstract
aims: This study was conducted to determine optimal conditions for pectate lyase (PL) production by two psychrophilic yeast strains and to compare the properties of the cold-active enzymes using mesophilic PL as reference enzyme.
methods and results: Two psychrophilic yeasts isolated from remote geographical locations (European Alps, north Siberia) produced extracellular cold-active PL. Both strains were identified as Mrakia frigida by analysis of ITS and large subunit (LSU) rRNA sequences. Maximum enzyme production occurred at a cultivation temperature of 1 or 5 degrees C. The apparent optimum for enzyme activity was observed at 30 degrees C and pH 8.5-9. The enzymes were thermolabile, but were resistant to repeated freezing and thawing.
conclusion: We describe for the first time alkaline PL-producing representatives of the yeast species M. frigida. The two strains produce cold-active PL with similar properties, but have a different enzyme production pattern.
significance and impact of the study: The enzymes described in this study could be useful for a wide range of applications, such as low-temperature pretreatment of wastewater containing pectic substances.
MeSH terms
Basidiomycota; Cold Temperature; DNA, Fungal; DNA, Ribosomal; DNA, Ribosomal Spacer; Enzyme Stability; Genes, rRNA; Hydrogen-Ion Concentration; Molecular Sequence Data; Polysaccharide-Lyases; Sequence Analysis, DNA; Temperature
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