Differential protein expression by Porphyromonas gingivalis in response to secreted epithelial cell components.
Proteomics, 2005/1;5(1):198-211.
Zhang Y[1], Wang T, Chen W, Yilmaz O, Park Y, Jung IY, Hackett M, Lamont RJ
Affiliations
PMID: 15619293
Impact factor: 5.393
Abstract
The human oral pathogen Porphyromonas gingivalis colonizes the gingival crevice and invades gingival epithelial cells. Multidimensional capillary high-performance liquid chromatography coupled with tandem mass spectrometry and two-dimensional gel electrophoresis were used to analyze the proteome of P. gingivalis as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were identified in four independent analyses; 479 of these proteins showed evidence of differential expression after exposure of P. gingivalis to either conditioned epithelial cell growth medium or control conditions: i.e., they were only detected under one set of conditions. Moreover, 276 genes annotated as hypothetical were found to encode expressed proteins. Among the proteins up-regulated in the presence of epithelial cell components were a homolog of the internalin proteins of Listeria monocytogenes and subunits of the ATP-dependent Clp protease complex. Insertional inactivation of clpP, encoding the Clp proteolytic subunit, resulted in approximately a 50% reduction in invasion of P. gingivalis. These results suggest that adaptation to an epithelial cell environment induces a major shift in the expressed proteome of the organism. Furthermore, ClpP, that is up-regulated in this environment, is required for optimal invasive activity of P. gingivalis.
MeSH terms
Bacterial Adhesion; Bacterial Proteins; Cells, Cultured; Chromatography, High Pressure Liquid; Electrophoresis, Gel, Two-Dimensional; Endopeptidase Clp; Epithelial Cells; Gene Expression Profiling; Gingiva; Humans; Mass Spectrometry; Mutation; Porphyromonas gingivalis; Protein Array Analysis; Proteome; Up-Regulation
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download