Genotypic variation and identification of QTLs for agronomic traits, using AFLP and SSR markers in RILs of sunflower (Helianthus annuus L.).
Theor Appl Genet, 2004/11;109(7):1353-60.
Al-Chaarani GR[1], Gentzbittel L, Huang XQ, Sarrafi A
Affiliations
PMID: 15365625
Impact factor: 5.574
Abstract
A population of 77 recombinant inbred lines (RILs) were developed through single-seed descent from a cross between 'PAC-2' and 'RHA-266'. Seeds of the above-mentioned RILs and their parents were planted in the field in a randomised complete block design with two replications. Genetic control for some agronomical traits-sowing-to-flowering date, plant height, stem diameter (SD), head diameter (HD), grain weight per plant, 1,000-grain weight (TGW) and the percentage of oil in grains-were measured for RILs and their parents. Genetic variability was observed among 77 RILs for all traits studied. Transgressive segregation occurred for some traits, and the comparison between 10% of selected RILs with the best parent showed significant difference for SD and HD as well as for TGW. A set of 123 RILs from the same cross, including the 77 above-mentioned RILs and their two parents, were screened with 409 AFLP and SSR markers, and a linkage map was constructed based on 367 markers. Several QTLs associated with the studied traits were identified. The effects of each QTL are moderate, ranging from 7% to 37%, but a high percentage of phenotypic variance is explained when considering all the covariants (TR2 mean around 80% in each trait). Although the detected regions need to be more precisely mapped, the information obtained should help in marker-assisted selection.
MeSH terms
Analysis of Variance; Chromosome Mapping; Chromosomes, Plant; Crosses, Genetic; Genetic Markers; Genetic Variation; Helianthus; Quantitative Trait Loci
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