Large-scale gene expression analysis of human skeletal myoblast differentiation.
Neuromuscul Disord, 2004/9;14(8-9):507-18.
Sterrenburg E[1], Turk R, 't Hoen PA, van Deutekom JC, Boer JM, van Ommen GJ, den Dunnen JT
Affiliations
PMID: 15336692
Impact factor: 3.538
Abstract
To study pathways involved in human skeletal myogenesis, we profiled gene expression in human primary myoblast cells derived from three individuals using both oligonucleotide and cDNA microarrays. Following stringent statistical testing (false-positive rate 0.4%), we identified 146 genes differentially expressed over time. Interestingly, 86 of these genes have not been reported to be involved in myogenesis in mouse cell lines. This demonstrates the additional value of human primary cell cultures in the study of muscle differentiation. Many of the identified genes play a role in muscle regeneration, indicating the close relationship of this process with muscle development. In addition, we found overlap with expression profiling studies in muscle from Duchenne muscular dystrophy patients, confirming ongoing muscle regeneration in Duchenne muscular dystrophy. Further study of these genes can bring new insights into the process of muscle differentiation, and they are candidate genes for neuromuscular disorders with an as yet unidentified cause.
MeSH terms
Cell Differentiation; Cells, Cultured; Cluster Analysis; DNA-Binding Proteins; Desmin; Gene Expression Profiling; Humans; Immunohistochemistry; Indoles; Laminin; Microfilament Proteins; Muscle Proteins; Myoblasts, Skeletal; Myogenic Regulatory Factor 5; Myosins; Oligonucleotide Array Sequence Analysis; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Trans-Activators
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