Halobacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine Permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description of H. salinarum.
Extremophiles, 2004/12;8(6):431-9.
Gruber C[1], Legat A, Pfaffenhuemer M, Radax C, Weidler G, Busse HJ, Stan-Lotter H
Affiliations
PMID: 15290323
Impact factor: 3.035
Abstract
Two rod-shaped haloarchaeal strains, A1 and A2, were isolated from a bore core from a salt mine in Austria. The deposition of the salt is thought to have occurred during the Permian period (225-280 million years ago). The 16S rDNA sequences of the strains were 97.1% similar to that of the type species of the genus Halobacterium, which was also determined in this work. Polar lipids consisted of C20-C20 derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and sulfated tetraglycosyl diether. Optimal salinity for growth was 15-17.5% NaCl; Mg++ was tolerated up to a concentration of 1 M. The DNA-DNA reassociation value of strain A1T was 25% with H. salinarum DSM 3754T and 41% with Halobacterium sp. NRC-1, respectively. Based on these results and other properties, e.g. whole cell protein patterns, menaquinone content and restriction patterns of DNA, strains A1 and A2 are members of a single species, for which we propose the name H. noricense. The type strain is A1 (DSM 15987T, ATCC BAA-852T, NCIMB 13967T). Since we present evidence that Halobacterium sp. NRC-1 is a member of H. salinarum, an emended description of H. salinarum is provided.
MeSH terms
Archaeal Proteins; Austria; Base Composition; Carotenoids; DNA, Archaeal; DNA, Ribosomal; Genes, Archaeal; Geologic Sediments; Halobacterium; Halobacterium salinarum; Lipids; Microscopy, Electron, Scanning; Phylogeny; Plasmids; RNA, Archaeal; RNA, Ribosomal, 16S; Sodium Chloride; Species Specificity; Terminology as Topic; Vitamin K 2
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