Growth factor regulation of human growth plate chondrocyte proliferation in vitro.
Biochem Biophys Res Commun, 2004/5/14;317(4):1171-82.
Olney RC[1], Wang J, Sylvester JE, Mougey EB
Affiliations
PMID: 15094393
Impact factor: 3.322
Abstract
Linear growth occurs as the result of growth plate chondrocytes undergoing proliferative and hypertrophic phases. Paracrine feedback loops that regulate the entry of chondrocytes into the hypertrophic phase have been shown and similar pathways likely exist for the proliferative phase. Human long-bone growth plate chondrocytes were cultured in vitro. The proliferative effects of a variety of factors were determined by [3H]thymidine uptake and the gene expression profile of these cells was determined by DNA microarray analysis. Serum, insulin-like growth factor (IGF)-I and -II, transforming growth factor-beta (TGF-beta, fibroblast growth factor (FGF)-1, -2, and -18, and platelet-derived growth factor (PDGF)-BB were potent stimulators of proliferation. FGF-10, testosterone, and bone morphogenetic proteins (BMP)-2, -4, and -6 inhibited proliferation. Microarray analysis showed that the genes for multiple members of the IGF-I, TGF-beta, FGF, and BMP pathways were expressed, suggesting the presence of autocrine/paracrine pathways that regulate the proliferative phase of growth plate-mediated growth.
MeSH terms
Adolescent; Animals; Cattle; Cell Division; Cells, Cultured; Child; Chondrocytes; Collagen; Dose-Response Relationship, Drug; Extracellular Matrix; Female; Fetal Blood; Gene Expression Profiling; Growth Plate; Growth Substances; Hormones; Humans; Male; Protein-Tyrosine Kinases; RNA, Messenger; Receptor, Fibroblast Growth Factor, Type 3; Receptors, Fibroblast Growth Factor; Thymidine
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