L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.
Appl Environ Microbiol, 2004/3;70(3):1869-73.
van der Kaaij H[1], Desiere F, Mollet B, Germond JE
Affiliations
PMID: 15006820
Impact factor: 5.005
Abstract
Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.
MeSH terms
Alanine; Alanine Dehydrogenase; Amino Acid Oxidoreductases; Bacillus subtilis; Base Sequence; DNA, Bacterial; Genetic Complementation Test; Genome, Bacterial; Lactobacillus
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