Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism.
EMBO J, 1992/2;11(2):611-8.
Usuda S[1], Takemori T, Matsuoka M, Shirasawa T, Yoshida K, Mori A, Ishizaka K, Sakano H
Affiliations
PMID: 1311252
Impact factor: 14.012
Abstract
Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
MeSH terms
Animals; B-Lymphocytes; Base Sequence; Cell Line, Transformed; Chromosome Deletion; DNA; DNA Probes; DNA, Circular; Female; Genes, Immunoglobulin; Immunoglobulin Heavy Chains; Immunoglobulin Variable Region; Leukemia Virus, Murine; Lymph Nodes; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Oligodeoxyribonucleotides; Restriction Mapping; Sequence Homology, Nucleic Acid; Spleen
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