Characterization of a MYBR2R3 gene from black spruce (Picea mariana) that shares functional conservation with maize C1.
Mol Genet Genomics, 2003/10;270(1):78-86.
Xue B[1], Charest PJ, Devantier Y, Rutledge RG
Affiliations
PMID: 12920576
Impact factor: 2.98
Abstract
PCR amplification with degenerate primers targeted to highly conserved amino acid motifs within the MYB domain was used to demonstrate that black spruce (Picea mariana) possesses a diverse MYB gene family. Amino acid sequence comparisons revealed three broad MYB subfamilies, one of which shares extensive similarity with maize C1, a central regulator of anthocyanin biosynthesis. A cDNA clone encoding a MYBR2R3 protein from P. mariana with high levels of sequence homology to maize C1 was shown to transactivate the Bz2 promoter in combination with maize R in embryonal tissues of both black spruce and larch. Functional dependence on the maize R protein, and the presence of a conserved C-terminal GIDPxTH motif, support the conservation of MYBR2R3 function in conifers, and demonstrate that the basic components of MYBR2R3-dependent transcriptional regulation have been conserved between angiosperms and gymnosperms.
MeSH terms
Amino Acid Sequence; Base Sequence; Conserved Sequence; DNA Primers; DNA-Binding Proteins; Gene Expression Regulation; Molecular Sequence Data; Multigene Family; Phylogeny; Picea; Plant Proteins; Polymerase Chain Reaction; Seeds; Sequence Homology, Amino Acid; Trees; Zea mays
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