Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation.
Antimicrob Agents Chemother, 2003/8;47(8):2565-71.
Galimand M[1], Courvalin P, Lambert T
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PMID: 12878520
Impact factor: 5.938
Abstract
A self-transferable plasmid of ca. 80 kb, pIP1204, conferred multiple-antibiotic resistance to Klebsiella pneumoniae BM4536, which was isolated from a urinary tract infection. Resistance to beta-lactams was due to the bla(TEM1) and bla(CTX-M) genes, resistance to trimethroprim was due to the dhfrXII gene, resistance to sulfonamides was due to the sul1 gene, resistance to streptomycin-spectinomycin was due to the ant3"9 gene, and resistance to nearly all remaining aminoglycosides was due to the aac3-II gene and a new gene designated armA (aminoglycoside resistance methylase). The cloning of armA into a plasmid in Escherichia coli conferred to the new host high-level resistance to 4,6-disubstituted deoxystreptamines and fortimicin. The deduced sequence of ArmA displayed from 37 to 47% similarity to those of 16S rRNA m(7)G methyltransferases from various actinomycetes, which confer resistance to aminoglycoside-producing strains. However, the low guanine-plus-cytosine content of armA (30%) does not favor an actinomycete origin for the gene. It therefore appears that posttranscriptional modification of 16S rRNA can confer high-level broad-range resistance to aminoglycosides in gram-negative human pathogens.
MeSH terms
Amino Acid Sequence; Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae; Klebsiella pneumoniae; Methylation; Methyltransferases; Microbial Sensitivity Tests; Molecular Sequence Data; Plasmids; RNA, Ribosomal, 16S
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