Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions.
FEMS Yeast Res, 2002/12;2(4):495-517.
Scorzetti G[1], Fell JW, Fonseca A, Statzell-Tallman A
Affiliations
PMID: 12702266
Impact factor: 2.923
Abstract
Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5' end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenetic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization.
MeSH terms
Basidiomycota; DNA, Fungal; DNA, Ribosomal; DNA, Ribosomal Spacer; Genetic Variation; Phylogeny; Sequence Analysis, DNA
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