[Production and some properties of a thermophilic protease from Bacillus stearothermophilus WF146].
Wei Sheng Wu Xue Bao, 2000/4;40(2):188-92.
Tang B[1], Zhou L, Chen X, Dai X, Peng Z
Affiliations
PMID: 12548943
Abstract
The factors affecting Bacillus stearothermophilus WF146 for thermophilic protease producing have been investigated, more than 600 units of enzyme in 1 mL of fermented culture could be achieved under suitable condition. The protease had a molecular weight around 34 kD estimated by SDS-PAGE, and functioned optimally at pH 8.0 and 80 degrees C, respectively. In addition, the enzyme exhibited high temperature tolerance and was stable at a wide range of pH, and Ca2+ played a key role for the stability of the enzyme. While the protease activity of the enzyme was strongly inhibited by PMSF, DFP and IAA, and was not affected by DTT.
MeSH terms
Endopeptidases; Enzyme Stability; Fermentation; Geobacillus stearothermophilus; Hydrogen-Ion Concentration; Temperature
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