Worldwide distribution of Nitrosococcus oceani, a marine ammonia-oxidizing gamma-proteobacterium, detected by PCR and sequencing of 16S rRNA and amoA genes.
Appl Environ Microbiol, 2002/8;68(8):4153-7.
Ward BB[1], O'Mullan GD
Affiliations
PMID: 12147525
Impact factor: 5.005
Abstract
Diversity of cultured ammonia-oxidizing bacteria in the gamma-subdivision of the Proteobacteria was investigated by using strains isolated from various parts of the world ocean. All the strains were very similar to each other on the basis of the sequences of both the 16S rRNA and ammonia monooxygenase genes and could be characterized as a single species. Sequences were also cloned directly from environmental DNA from coastal Pacific and Atlantic sites, and these sequences represented the first Nitrosococcus oceani-like sequences obtained directly from the ocean. Most of the environmental sequences clustered tightly with those of the cultivated strains, but some sequences could represent new species of NITROSOCOCCUS: These findings imply that organisms similar to the cultivated N. oceani strains have a worldwide distribution.
MeSH terms
Ammonia; Atlantic Ocean; DNA, Ribosomal; Gammaproteobacteria; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases; Pacific Ocean; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Seawater
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