3-Chloro-DL-alanine resistance by L-methionine-alpha-deamino-gamma-mercaptomethane-lyase activity.
FEBS Lett, 2002/7/17;523(1-3):119-22.
Yoshimura M[1], Nakano Y, Fukamachi H, Koga T
Affiliations
PMID: 12123816
Impact factor: 3.864
Abstract
The antibacterial agent 3-chloro-DL-alanine (3CA) is an inhibitor of peptidoglycan synthesis. Fusobacterium nucleatum and Porphyromonas gingivalis, the bacteria responsible for oral malodor, are shown to be resistant to 1 mM 3CA, whereas Streptococcus mutans and Escherichia coli are sensitive to this antibacterial agent at the same concentration. We isolated the 3CA resistance gene from F. nucleatum and showed that the gene encodes an L-methionine-alpha-deamino-gamma-mercaptomethane-lyase that catalyzes the alpha,gamma-elimination of L-methionine to produce methyl mercaptan. The enzyme also exhibits 3CA chloride-lyase (deaminating) activity. This antibacterial agent is expected to be useful for specific selection of malodorous oral bacteria producing high amounts of methyl mercaptan.
MeSH terms
Anti-Bacterial Agents; Carbon-Sulfur Lyases; Deamination; Drug Resistance, Bacterial; Fusobacterium nucleatum; Methionine; Microbial Sensitivity Tests; Porphyromonas gingivalis; Species Specificity; Sulfhydryl Compounds; beta-Alanine
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