Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain.
Antimicrob Agents Chemother, 2002/6;46(6):2014-6.
Iyobe S[1], Kusadokoro H, Takahashi A, Yomoda S, Okubo T, Nakamura A, O'Hara K
Affiliations
PMID: 12019129
Impact factor: 5.938
Abstract
The gene bla(IMP-10) of a variant metallo-beta-lactamase, IMP-10, had a single base replacement of G by T at nucleotide 145, which led to an amino acid alteration of Val49 to Phe compared to the IMP-1 enzyme, indicating that IMP-10 was a point mutation derivative of IMP-1. Highly purified enzymes revealed that IMP-10 was different from IMP-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin.
MeSH terms
Alcaligenes; DNA, Bacterial; Drug Resistance, Microbial; Imipenem; Kinetics; Plasmids; Point Mutation; Pseudomonas aeruginosa; Reverse Transcriptase Polymerase Chain Reaction; Spectrophotometry, Ultraviolet; Thienamycins; beta-Lactamases
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download