Two distinct isopentenyl diphosphate isomerases in cytosol and plastid are differentially induced by environmental stresses in tobacco.
FEBS Lett, 2001/9/28;506(1):61-4.
Nakamura A[1], Shimada H, Masuda T, Ohta H, Takamiya K
Affiliations
PMID: 11591371
Impact factor: 3.864
Abstract
Two distinct cDNA clones (IPI1 and IPI2) encoding IPI were isolated from Nicotiana tabacum. In situ expression of isopentenyl diphosphate isomerase-1 (IPI1)- and IPI2-green fluorescent protein fusion constructs revealed that IPI1 and IPI2 were localized in chloroplast and cytosol, respectively. The level of IPI1 mRNA was increased under high-salt and high-light stress conditions, while that of IPI2 mRNA was increased under high-salt and cold stress conditions. Both IPI transcripts were increased in an abscisic acid-independent manner. This is the first report of a cytosolic IPI. The results indicated that two distinct IPIs were differentially induced in response to stress.
MeSH terms
Abscisic Acid; Base Sequence; Carbon-Carbon Double Bond Isomerases; Cytosol; DNA Primers; Green Fluorescent Proteins; Hemiterpenes; Isoenzymes; Luminescent Proteins; Molecular Sequence Data; Plants, Toxic; Plastids; Recombinant Fusion Proteins; Subcellular Fractions; Nicotiana
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