Outbreak of infection with extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a Mexican hospital.
J Clin Microbiol, 2001/9;39(9):3193-6.
Silva J[1], Gatica R, Aguilar C, Becerra Z, Garza-Ramos U, Velázquez M, Miranda G, Leaños B, Solórzano F, Echániz G
Affiliations
PMID: 11526149
Impact factor: 11.677
Abstract
Thirty-one strains of Klebsiella pneumoniae (including 10 duplicates) from 21 septicemic pediatric patients (age, <2 months) were studied during a 4-month period (June to October 1996) in which the fatality rate was 62% (13 of 21). These isolates identified by the API 20E system yielded the same biotype. Pulsed-field gel electrophoresis experiments revealed the same clone in 31 strains. The isolates were multidrug-resistant but were still susceptible to ciprofloxacin, imipenem, and cefoxitin. A 135-kb plasmid was harbored in all of the isolates. No transconjugants were obtained that were resistant to ampicillin, cefotaxime, tetracycline, or gentamicin. Isoelectric focusing for beta-lactamases was performed on all strains, and three bands with pIs of 5.4, 7.6, and 8.2 were obtained. Of these, the pI 8.2 beta-lactamase had an extended-spectrum beta-lactamase phenotype. PCR amplification of both TEM- and SHV-type genes was obtained. The sequence analysis of the SHV PCR product indicated a mutation corresponding to the SHV-5 beta-lactamase.
MeSH terms
Bacteremia; Bacterial Typing Techniques; Cross Infection; Disease Outbreaks; Drug Resistance, Bacterial; Hospitals; Humans; Infant; Infant, Newborn; Klebsiella Infections; Klebsiella pneumoniae; Mexico; Microbial Sensitivity Tests; beta-Lactamases; beta-Lactams
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