LldI, a plasmid-encoded type I restriction and modification system in Lactococcus lactis.
DNA Seq, 2000;11(3-4):239-45.
Affiliations
PMID: 11092734
Abstract
A plasmid-encoded type I restriction and modification (R-M) system, designated LldI, was identified in Lactococcus lactis biovar diacetylactis LD10-1. LldI consists of three genes encoding endonuclease, methylase and specificity subunits, respectively. RT-PCR analysis revealed that the three genes are co-transcribed as a polycistronic mRNA in L. lactis. The specificity subunit of LldI differs significantly in the target recognition domains from those of other type I R-M systems, suggesting that LldI confers a novel specificity in L. lactis.
MeSH terms
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Conserved Sequence; DNA Restriction Enzymes; Deoxyribonucleases, Type I Site-Specific; Genes, Bacterial; Lactococcus lactis; Molecular Sequence Data; Plasmids; Protein Subunits; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Homology, Amino Acid; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transcription, Genetic
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