Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon.
Microbiology (Reading), 1999/11;145 ( Pt 11):3155-3161.
Martı Nez B[1], Fernández MA[2], Suárez JE[2, 1], Rodrı Guez A[1]
Affiliations
PMID: 10589723DOI: 10.1099/00221287-145-11-3155
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Abstract
Synthesis of lactococcin 972 is plasmid-encoded. An operon composed of two genes that encode pre-bacteriocin and a putative immunity protein has been identified. The first gene encodes a 91-residue polypeptide that is exported via a sec-dependent system to give the mature 66-aa bacteriocin. The immunity protein is a 563-residue polypeptide with seven potential transmembrane domains. Two transcripts were observed from this region: one comprises the whole operon and is synthesized during the exponential phase of growth while the other, which corresponds just to the bacteriocin structural gene, presents a maximum in exponential cultures but is still present in late-stationary-phase cells.
MeSH terms
Amino Acid Sequence; Bacteriocins; Base Sequence; Blotting, Northern; Electrophoresis, Polyacrylamide Gel; Genes, Bacterial; Lactococcus lactis; Molecular Sequence Data; Operon; Plasmids; RNA, Bacterial; Time Factors
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