Isolation and characterization of the E2F-like gene in plants.
FEBS Lett, 1999/10/22;460(1):117-22.
Sekine M[1], Ito M, Uemukai K, Maeda Y, Nakagami H, Shinmyo A
Affiliations
PMID: 10571072
Impact factor: 3.864
Abstract
The transcription factor E2F regulates the expression of genes involved in the progression of G1/S transition and DNA replication in mammalian cells. We cloned and characterized a cDNA (NtE2F) corresponding to a E2F homolog of tobacco (Nicotiana tabacum). The transcription of NtE2F was induced as cells progressed from G1 to the S phase and expressed much earlier than that of the proliferating cell nuclear antigen (PCNA) gene. We demonstrated that NtE2F can interact with the tobacco retinoblastoma (Rb)-related protein in a yeast two-hybrid assay. To further characterize NtE2F, the trans-activation activity of NtE2F was examined by using a transient assay in the tobacco Bright Yellow-2 (BY-2) cells with NtE2F fused to the DNA-binding domain of the veast transcriptional activator GAL4. NtE2F activated the transcription of the beta-glucuronidase (GUS) reporter gene driven by a cauliflower mosaic virus (CaMV) 35S core promoter containing the GAL4-binding sequence. This is the first report of the identification of a functionally equivalent E2F-like gene in plants.
MeSH terms
Amino Acid Sequence; Carrier Proteins; Cell Cycle Proteins; Cell Line; Cloning, Molecular; DNA-Binding Proteins; E2F Transcription Factors; Gene Expression Regulation, Plant; Genes, Plant; Genes, Reporter; Molecular Sequence Data; Plants, Toxic; Proliferating Cell Nuclear Antigen; RNA, Messenger; Retinoblastoma Protein; Retinoblastoma-Binding Protein 1; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Nicotiana; Transcription Factors; Yeasts
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