Mapping studies and genetic analysis of transfer genes of the multi-resistant IncHI2 plasmid, R478.
FEMS Microbiol Lett, 1999/10/01;179(1):21-9.
Page DT[1], Whelan KF, Colleran E
Affiliations
PMID: 10481081
Impact factor: 2.82
Abstract
A bank of transfer-defective Tn7 insertion mutants of the multi-resistant IncHI2 megaplasmid, R478, was generated. Complementation analysis of these mutations identified a large 144-kb transfer-associated region of R478. A 6.8-kb segment from the transfer region was sequenced. The precise locations of Tn7 insertion within four distinct R478::Tn7 transfer-defective mutants were mapped and each insertion was found to disrupt a specific open reading frame. These transfer-associated determinants of R478 were designated htdB (H transfer determinant), htdD, htdT and htdC. Both htdB and htdC encoded amino acid sequences that showed a low homology with pilus biosynthetic proteins encoded by the F plasmid.
MeSH terms
Amino Acid Sequence; Bacterial Proteins; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli; Fimbriae Proteins; Genetic Complementation Test; Molecular Sequence Data; Mutagenesis, Insertional; R Factors; Restriction Mapping; Sequence Alignment; Sequence Homology, Amino Acid
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