The effect of moxifloxacin on its target topoisomerases from Escherichia coli and Staphylococcus aureus.
J Antimicrob Chemother, 1999/5;43 Suppl B:31-7.
Schedletzky H[1], Wiedemann B, Heisig P
Affiliations
PMID: 10382873
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Abstract
The effect of moxifloxacin on its target enzymes was evaluated by three different approaches: (i) the MICs of moxifloxacin and nine other fluoroquinolones were determined for mutants of Escherichia coli (n = 13) and Staphylococcus aureus (n = 5) carrying different combinations of resistance mutations; (ii) the activity of moxifloxacin on isolated targets was determined as IC50 values for wild-type and mutant type II topoisomerases from E. coli; and (iii) the mutation frequencies were determined for two single-step mutants (MI with a Ser83-->Leu mutation in gyrA and WT-4 with a Ser80-->Ile mutation in parC) and their parent strain (WT). Of the quinolones tested, moxifloxacin was the only one showing an equivalent high activity against both targets. This is reflected by a comparable high susceptibility of the test strains of E. coli and S. aureus and by the IC50 values of moxifloxacin which were 50-90% lower than those of ciprofloxacin, norfloxacin and sparfloxacin for the wild-type and single mutant enzymes of gyrase and topoisomerase IV. However, double mutant GyrA was significantly more sensitive to moxifloxacin than to the other fluoroquinolones tested, while wild-type topoisomerase IV was two-fold more refractory. Mutation rates of WT, MI and WT-4 for ciprofloxacin and moxifloxacin were 5 x 10(-8) vs 4 x 10(-10); <6 x 10(-11) vs <6 x 10(-11); and 2 x 10(-6) vs 5 x 10(-7), respectively. These data indicate an equivalent high inhibitory activity of moxifloxacin on DNA gyrase and topoisomerase IV of E. coli.
MeSH terms
Anti-Infective Agents; Aza Compounds; DNA Gyrase; DNA Topoisomerase IV; DNA Topoisomerases, Type I; DNA Topoisomerases, Type II; Drug Resistance, Microbial; Escherichia coli; Fluoroquinolones; Genes, Bacterial; Microbial Sensitivity Tests; Moxifloxacin; Mutation; Quinolines; Staphylococcus aureus
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