Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification.
J Clin Microbiol, 1999/6;37(6):1985-93.
Sugita T[1], Nishikawa A, Ikeda R, Shinoda T
Affiliations
PMID: 10325360
Impact factor: 11.677
Abstract
The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species, T. asahii, T. asteroides, T. cutaneum, T. inkin, T. mucoides, and T. ovoides, can be readily identified by their ITS sequences. In addition, the sequence analysis showed that conspecific strains have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall. Molecular phylogenetic trees are also presented.
MeSH terms
Base Sequence; Candida albicans; DNA, Fungal; DNA, Ribosomal; Databases as Topic; Evolution, Molecular; Japan; Molecular Sequence Data; Phylogeny; Saccharomyces cerevisiae; Sequence Alignment; Sequence Homology, Nucleic Acid; Soil Microbiology; Transcription, Genetic; Trichosporon
More resources
Full text:
Europe PubMed Central; PubMed Central
EndNote: Download