Sequence analysis of a 32-kb region including the major ribosomal protein gene clusters from alkaliphilic Bacillus sp. strain C-125.
Biosci Biotechnol Biochem, 1999/2;63(2):452-5.
Takami H[1], Takaki Y, Nakasone K, Hirama C, Inoue A, Horikoshi K
Affiliations
PMID: 10192928
Impact factor: 2.337
Abstract
Forty-one open reading frames (ORFs) were identified in a 32-kb DNA fragment of alkaliphilic Bacillus sp. C-125. A similarity search using the BSORF database found 37 ORFs with significant sequence similarity to B. subtilis RNA polymerase subunits, elongation factor G, elongation factor Tu, and ribosomal proteins. Each ORF product showed more than 70% identity to those of B. subtilis. Gene organization in the region of str, S10, spc, and the alpha cluster was highly conserved among three strains, C-125, B. subtilis, and B. stearothermophilus.
MeSH terms
Bacillus; Base Sequence; Chromosome Mapping; Cloning, Molecular; Conserved Sequence; DNA Primers; DNA, Bacterial; Molecular Sequence Data; Multigene Family; Open Reading Frames; Polymerase Chain Reaction; Ribosomal Proteins; Sequence Analysis, DNA
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