A plasmid-encoded two-component regulatory system involved in copper-inducible transcription in Lactococcus lactis.
Gene, 1999/3/18;229(1-2):229-35.
Khunajakr N[1], Liu CQ, Charoenchai P, Dunn NW
Affiliations
PMID: 10095123
Impact factor: 3.913
Abstract
Two regulatory genes (lcoR and lcoS) were identified from a plasmid-borne lactococcal copper resistance determinant and characterized by transcriptional fusion to the promoterless chloramphenicol acetyltransferase gene (cat). RT-PCR analysis indicates that lcoR and lcoS are organized within an operon, controlling the transcription of cat in a copper-inducible manner. The amino acid sequences deduced from lcoR and lcoS show homology to the response and sensor proteins of known two-component regulatory systems. Deletion within either lcoS or both genes inactivated the copper-dependent activity, suggesting the presence of no trans-acting lcoR and lcoS homologs in the lactococcal host chromosome. The transcription start site involved in copper induction was mapped by primer extension.
MeSH terms
Base Sequence; Cloning, Molecular; Copper; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Reporter; Lactococcus lactis; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; RNA, Messenger; Sequence Deletion; Transcription, Genetic
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