Nephritogenicity and alpha-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane.
Virchows Arch, 1996/7;428(4-5):281-8.
Rauf S[1], Kagawa M, Kishiro Y, Inoue S, Naito I, Oohashi T, Sugimoto M, Ninomiya Y, Sado Y
Affiliations
PMID: 8764938
Impact factor: 4.535
Abstract
Nephritogenicity (anti-GBM-nephritis-inducing activity) and alpha-chain composition of globular-do-main (NCI) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different alpha chains of type IV collagen. A purified nephritogenic fraction from renal BM contained alpha 1-alpha 6(IV)NCI, whereas a non-nephritogenic fraction contained only alpha 1-alpha 2(IV)NCI. Renal and pulmonary NCI had strong nephritogenic activity: placental NCI had weak activity. The renal and pulmonary fractions contained alpha 1-alpha 6(IV)NCI, and the placental fraction had a large amount of alpha 1-alpha 2(IV)NCI and a very small amount of alpha 3-alpha 6(IV)NCI. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained alpha 1-alpha 5(IV) chains, but not the alpha 6(IV) chain. The absence of alpha 6(IV) chain in glomerular BM in bovine and in humans indicates that alpha 6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of alpha 3-alpha 5(IV)NCI.
MeSH terms
Animals; Antibodies, Monoclonal; Autoantigens; Autoimmune Diseases; Basement Membrane; Blotting, Western; Cattle; Collagen; Female; Fluorescent Antibody Technique, Indirect; Glomerulonephritis; Immunoglobulin G; Immunohistochemistry; Kidney Glomerulus; Lung; Rats; Rats, Inbred WKY
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