Scaffolds of bioactive glass-ceramic (Biosilicate®) and bone healing: A biological evaluation in an experimental model of tibial bone defect in rats.
Biomed Mater Eng, 2018;29(5):665-683.
Pinto KNZ[1], Tim CR[2, 3], Crovace MC[4], Rossi BRO[5], Kido HW[3, 6], Parizotto NA[2, 7], Zanotto ED[4], Peitl O[4], Rennó AC[3]
Affiliations
PMID: 30400079DOI: 10.3233/BME-181016
Impact factor: 1.234
Abstract
This study aimed to investigate the in vivo tissue response of the Biosilicate® scaffolds in a model of tibial bone defect. Sixty male Wistar rats were distributed into bone defect control group (CG) and Biosilicate® scaffold group (BG). Animals were euthanized 15, 30 and 45 days post-surgery. Stereomicroscopy, scanning electron microscopy, histopathological, immunohistochemistry and biomechanical analysis were used. Scaffolds had a total porosity of 44%, macroporosity of 15% with pore diameter of 230 μm. Higher amount of newly formed bone was observed on days 30 and 45 in BG. Immunohistochemistry analysis showed that the COX-2 expression was significantly higher on days 15 and 30 in BG compared with the CG. RUNX-2 immunoexpression was significantly higher in BG on days 15 and 45. No statistically significant difference was observed in RANKL immunoexpression in all experimental groups. BMP-9 immunoexpression was significantly upregulated in the BG on day 45. Biomechanical analysis showed a decrease in the biomechanical properties of the bone callus on days 30 and 45. The implantation of the Biosilicate® scaffolds was effective in stimulating newly bone formation and produced an increased immunoexpression of markers related to the bone repair.
Keywords: Biosilicate®; bone repair; glass-ceramic; rats; scaffold
MeSH terms
Animals; Biomechanical Phenomena; Bone Substitutes; Fracture Healing; Glass; Male; Osteogenesis; Rats; Rats, Wistar; Tibia; Tibial Fractures; Tissue Scaffolds
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