Cartilage acidic protein-1B (LOTUS), an endogenous Nogo receptor antagonist for axon tract formation.
Science, 2011/8/05;333(6043):769-73.
Sato Y[1], Iketani M, Kurihara Y, Yamaguchi M, Yamashita N, Nakamura F, Arie Y, Kawasaki T, Hirata T, Abe T, Kiyonari H, Strittmatter SM, Goshima Y, Takei K
Affiliations
PMID: 21817055DOI: 10.1126/science.1204144
Impact factor: 63.714
Abstract
Neural circuitry formation depends on the molecular control of axonal projection during development. By screening with fluorophore-assisted light inactivation in the developing mouse brain, we identified cartilage acidic protein-1B as a key molecule for lateral olfactory tract (LOT) formation and named it LOT usher substance (LOTUS). We further identified Nogo receptor-1 (NgR1) as a LOTUS-binding protein. NgR1 is a receptor of myelin-derived axon growth inhibitors, such as Nogo, which prevent neural regeneration in the adult. LOTUS suppressed Nogo-NgR1 binding and Nogo-induced growth cone collapse. A defasciculated LOT was present in lotus-deficient mice but not in mice lacking both lotus- and ngr1. These findings suggest that endogenous antagonism of NgR1 by LOTUS is crucial for normal LOT formation.
MeSH terms
Animals; Axons; Binding Sites; Calcium-Binding Proteins; Cell Line; Cells, Cultured; GPI-Linked Proteins; Growth Cones; Humans; Immunohistochemistry; Ligands; Mice; Mice, Inbred ICR; Myelin Proteins; Nogo Proteins; Nogo Receptor 1; Olfactory Pathways; Prosencephalon; Protein Binding; Receptors, Cell Surface; Signal Transduction
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