Project name: Homo sapiens strain:A549

Description: Mitosis entails global and dramatical alterations such as nuclear envelope breakdown, chromatin condensation, higher-order chromatin organization disruption, concomitant with a global downregulation of transcription. How cells are able to faithfully re-establish the gene expression patterns upon mitotic exit and to maintain cellular identities remain poorly understood. Previous studies of mitotic chromatin features indicated that despite many transcriptional regulatory factors dissociate from mitotic chromatin, certain transcriptional regulatory factors especially transcription factors (TFs) remain associated with individual loci during mitosis and serve as mitotic bookmarking. However, it is still unclear exactly how transcriptional regulatory factors remain bound to compacted mitotic chromatin proteome-wide. In this study, we develop an approach called FAIRE-MS that combines FAIRE based open chromatin-associated protein pull-down and mass spectrometry (MS) to quantify the open chromatin-associated proteome during interphase and mitosis. We first perform FAIRE-seq on A549 cells in mitosis versus interphase to map chromatin accessibility genome-wide. It indicated that chromatin accessibility is largely maintained during mitosis, and distal cis-regulatory elements preserve accessibility more than promoters. Next, by using FAIRE-MS to enrich transcriptional regulatory factors, we were able to identify a total of 189 interphase and mitosis (IM) transcriptional regulatory factors. Notably, in our protein list, we not only identified mitotic bookmarking factors such as CEBPB, HMGB1, TFAP2A, but also found and demonstrated several factors, including MAX, HMGB3, hnRNP A2/B1, FUS, hnRNP D and TAIL1, are at least partially bound to mitotic chromatin. Furthermore, in the future, it will be necessary to study the role of these candidate proteins during the cell cycle. In addition, our data also provide a rich resource for further investigation of mitotic bookmarkers and its molecular mechanisms.

Data type: genome sequencing

Sample scope: Monoisolate

Relevance: Other

Last updated: 2022-04-01